Spatial Representativeness of Environmental DNA Metabarcoding Signal for Fish Biodiversity Assessment in a Natural Freshwater System
Fig 2
First factorial map of the correspondence analysis based on the whole dataset.
Color codes for the different localities sampled in 2014 using eDNA metabarcoding (dots): lake (L, green), impoundment (IM1 and IM2, red), Tier River (R0: dark blue, R1: light blue, R2; pink). The results of the different filtrations are indicated by a letter following the site name: three for L (a-c) and R0 (a-c), six for R1 (a-f) and R2 (a-f). Traditional fish samples added as supplementary individuals (crosses for previous fishing sessions and triangles for simultaneous fishing sessions in 2014): green (Lake), dark blue (R0), light blue (R1), pink (R2) and orange colors (other river sites). The plain and dashed line ellipses define the area encircling 95% of the samples for a given site for the eDNA analysis and the combined traditional fishing campaigns, respectively. Bottom left, histogram of the inertia distribution per axis.