New Insight into Metal Ion-Driven Catalysis of Nucleic Acids by Influenza PA-Nter
Fig 2
Cleavage of short nucleic acids fragments.
Activity of PA-Nter on the various nucleic acids substrates in the presence of increasing concentrations of divalent metal ions. All samples were incubated with purified 2.55 μM PA-Nter at 25°C for 1 h and inactivated at 70°C for 5 min. (A) 6.8 μM 20-nt RNA, (B) 8.5 μM 19-nt DNA, (C) 2.4 μM 66-nt DNA. Possible secondary structures of these oligonucleotides are shown in S3 Fig.