Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2
Fig 7
Effect of Ani9 on endogenous CaCCs in PC3, Capan-1 and NHNE cells.
A) Immunoblot of ANO1 protein in FRT, FRT-ANO1, PC3, Capan-1 and NHNE cells. Blots shown are representative of experiments performed three times. B-C) Effect of Ani9 on CaCCs activity was measured in PC3 and Capan-1 cells expressing a halide sensor YFP. The indicated concentrations of Ani9 were applied 20 min prior to CaCCs activation by 100 μM ATP. D) Summary of dose response (mean ± S.E., n = 6). E) Effect of Ani9 on CaCCs activity in IL-4 treated (10 ng/mL; 48h) NHNE cells. CaCCs currents were induced by 100 μM ATP and then the indicated concentrations of Ani9 were applied. The remaining CaCCs currents were abolished by 100 μM tannic acid (TA). (right) Summary of dose response (mean ± S.E., n = 4).