Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2
Fig 5
Patch-clamp analysis of ANO1 inhibition by Ani9 in FRT-ANO1 cells.
A) Whole-cell ANO1 current was recorded at a holding potential at 0 mV and was pulsed to voltages between ± 100 mV (in steps of 20 mV) in the absence and presence of 50 nM, 100 nM and 1 μM Ani9. ANO1 was activated by 100 μM ATP. B) Current/voltage (I/V) plot of mean currents at the middle of each voltage pulse. C) The bar graphs summarize the current density data measured at + 80 mV (mean ± S.E., n = 4–6). ***P < 0.001.