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Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2

Fig 1

Identification of novel inhibitors of ANO1.

A) Chemical structures of ANO1 inhibitors. B) Apical membrane current measured in ANO1 expressing FRT cells in the presence of a transepithelial chloride gradient. Representative current traces showing Ani1, Ani7 and Ani9-induced ANO1 inhibition at the indicated concentrations. ANO1 was activated by 10 μM Eact, a specific activator of ANO1. The dashed gray line shows a control trace.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0155771.g001