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D-TRP(8)-γMSH Prevents the Effects of Endotoxin in Rat Skeletal Muscle Cells through TNFα/NF-KB Signalling Pathway

Fig 5

Effect of D-Trp(8)-γMSH (γMSH) treatment (500 μg/kg i.p.) on: phospho-FoxO1 (A), FoxO1 (B), phospho-FoxO3 (C) and FoxO3 (D), in gastrocnemius muscle of control rats and rats treated with LPS (250 μg/kg). PF = pair-fed rats. Proteins were measured by Western blotting with specific antibodies for total and phosphoprotein and expressed as percentage of the control rats treated with saline. Representative Western blots are shown at the right. Boxes with immunoblots represent spliced images based on group and treatment order. Data represent means ± SE (n = 7–10 rats). *P< 0.05 and **P< 0.01, vs. their respective control group. +P<0.05, ++P<0.01 vs. LPS-saline, °°P<0.01 vs. PF. LSD multiple comparisons test, following one way ANOVA.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0155645.g005