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Sox2 and Pax6 Play Counteracting Roles in Regulating Neurogenesis within the Murine Olfactory Epithelium

Fig 4

Sox2 transduction produces the largest clones while Pax6 produces the smallest.

(A-D) GFP expression in representative whole mounts, demonstrating typical clone size following transduction with EV, Sox2, Pax6, and SEP retroviral vectors, respectively. Scale bar in (D) corresponds to 50 μm and applies to all photos. (E) The range in cell number per clone is depicted as a box-and-whiskers scatter plot, and the median clone size is indicated by the horizontal black line within the box. The number of clones analyzed were as follows: EV– 49, Sox2–111, Pax6–150, and SEP– 165. Non-parametric ANOVA was statistically significant (Kruskal-Wallis, p < 0.05) as were post-hoc pairwise comparisons using Conover-Iman tests of multiple comparisons using rank sums; * designates p < 0.05.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0155167.g004