PI3K/AKT Signaling Pathway Is Essential for Survival of Induced Pluripotent Stem Cells
Fig 5
Decrease of membrane potential and increase of ROS production upon wortmannin treatment in AD-iPSCs.
(A) Decreased membrane potential of mitochondria was determined by flow cytometry after TMRM+ staining in AD-iPSCs. Cells were treated with different concentrations of wortmannin (1 μM, 2 μM, 4 μM) for different times (1 h, 2 h, 24 h). Treated cells (open graphs) were compared to untreated controls (gray). (B) The quantitative data represent mean values of triplicate experiments +/-SD. (C) The production of ROS was determined after H2DCFDA staining in AD-iPSCs treated with two different concentrations of wortmannin (2–4 μM) or 4 μM of MK-2206 at two different time points (2h, 6h), by flow cytometry. Treated cells (open graphs) were compared to untreated controls (gray). (D) Quantitative data represent the median values of treated cells compared to control cells. ROS production was depicted as fold-increase, the control was set to 1. Two independent experiments with triplicates revealed comparable results