Medium Renewal Blocks Anti-Proliferative Effects of Metformin in Cultured MDA-MB-231 Breast Cancer Cells
Fig 5
Low concentrations of 2-DG and metformin reduce viability of MDA-MB-231 cells synergistically.
(A) MDA-MB-231 cells were treated with metformin and 600 μM 2-DG for 72 hours in low-glucose (1 g/L) RPMI-1640. Medium was renewed daily. Viability was determined by MTS assay. Results are means±SEM (n = 3–4). *P≤0.05. (B) MDA-MB-231 cells were treated with metformin and 600 μM 2-DG for 72 hours in low-glucose RPMI-1640. Medium was renewed daily. Cell number was determined by Hoechst assay. Results are means±SEM (n = 3–4). *P≤0.05. (C) MDA-MB-231 cells were treated metformin and 600 μM 2-DG in low-glucose RPMI-1640 for 24 hours. Proliferation was determined by BrdU assay. Results are means±SEM (n = 3). *P≤0.05.