IDH1R132H in Neural Stem Cells: Differentiation Impaired by Increased Apoptosis
Fig 5
Expression of glial and neuronal markers by differentiated ebiNSc.
Double staining with antibodies to GFAP (red) and MAP2 (green) after 14 days of differentiation of non-transduced ebiNSc showing populations of astrocytic and neuronal cells. Photomicrographs demonstrate (A) Merged image of GFAP (red), MAP2 (green) and DAPI (blue) showing populations of GFAP and MAP2 positive cells with some overlap between the two markers; DAPI was used to estimate the percentage of cells positive for GFAP and MAP2; (B) GFAP (red); (C) MAP2 (green). The images at 200x magnification, scale bars mark 100μm.