Discriminating Intercalative Effects of Threading Intercalator Nogalamycin, from Classical Intercalator Daunomycin, Using Single Molecule Atomic Force Spectroscopy
Fig 6
AFM force-extension traces for daunomycin-treated dsDNA molecules, for the dsDNA:drug molar ratio (1:10) after (A) 1 h and (B) 48 h incubation; and for the molar ratio (1:26) after (C) 1 h and (D) 48 h incubation.
The red curves are the elasticity fits (well-fit to the high force ssDNA elasticity regions) according to the modified FJC model.