Discriminating Intercalative Effects of Threading Intercalator Nogalamycin, from Classical Intercalator Daunomycin, Using Single Molecule Atomic Force Spectroscopy
Fig 5
AFM force-extension traces for nogalamycin-treated dsDNA molecules, for dsDNA:drug molar ratio (1:10) after (A) 1 h and (B) 48 h incubation; for the molar ratio (1:26) after (C) 1 h and (D) 48 h incubation; and for the molar ratio 1:40 after (E) 1 h and (F) 48 h incubation.
The red curves are the elasticity fits (well-fit to the high force ssDNA elasticity regions) according to the modified FJC model.