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Phosphatidylinositol 3-Kinase/AKT Pathway Inhibition by Doxazosin Promotes Glioblastoma Cells Death, Upregulation of p53 and Triggers Low Neurotoxicity

Fig 2

Citotoxicity of doxazosin on C6 (A,C,E,G) and U138-MG (B,D,F,H) cells after treatment for 48 h.

MTT assay of C6 (A) and U138-MG (B). LDH activity of C6 (C) and U138-MG (D). SRB assay of C6 (E) and U138-MG (F). Data are represented by means±SEM (n = 4). ***p<0.001 compared to respective control, ANOVA followed by Tukey’s test. Confocal microscopy of C6 (G) and U138-MG (H). Photomicrographs are on top and SRB staining in the bottom. Magnification: 10X + 2,5.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0154612.g002