Interleukin-1β-Targeted Vaccine Improves Glucose Control and β-Cell Function in a Diabetic KK-Ay Mouse Model
Fig 4
IL-1β targeted vaccine (1βEPP) restores β-cell mass and protects from β-cell apoptosis.
(A) Triple staining for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (red), insulin (green), and DAPI (blue) was performed on fixed, paraffin-embedded pancreas sections. (B) β-cell mass per pancreas was estimated as the relative cross-sectional area of β-cells (determined by quantifying the cross-sectional area occupied by β-cells divided by the cross-sectional area of total tissue) multiplied by the weight of the pancreas. (C) β-cell apoptosis was quantified by percentage of TUNEL-positive β-cells. Ten sections from each pancreas spanning the width of the pancreas were included in the analysis. Data are shown as mean ± SEM. Compared with the KK-Ay control mice, *p < 0.05, **p < 0.01.