Cardiac Glycosides Activate the Tumor Suppressor and Viral Restriction Factor Promyelocytic Leukemia Protein (PML)
Fig 5
Loss of PML does not attenuate cell death induced by apoptotic stimuli.
Two PML knockout HEK293T cell clones (PML KO c1 and c2) were generated by CRISPR/Cas9 approach. A, PML protein levels were determined by immunofluorescence using anti-PML antibody in the control and 2 μM arsenic trioxide (As2O3)-treated wild type HEK293T (WT), as well as in two PML knockout clones (PML KO c1 and c2). B, Wild-type and knockout clones were cultured in 96 well plates at a density of 15,000/well. The next day, the cells were treated with 60 μM C2-ceramide, 300nM ouabain or 5 μM arsenic trioxide for 48h. Cell viability was determined using Cell Titer Glo assay. Data are mean ± SEM (n = 3).