Lactic Acid Fermentation of Cactus Cladodes (Opuntia ficus-indica L.) Generates Flavonoid Derivatives with Antioxidant and Anti-Inflammatory Properties
Fig 6
Intracellular reactive oxygen species (ROS) (fluorescence intensity units, Fi) (A) and Prostaglandin E2 (PGE2) (pg/ml) (B) on Caco-2/TC7 cells. Caco-2/TC7 cells were stimulated for 24 h at 37°C with IL-1β (25 ng/mL) and then treated for other 24 h with water-soluble (WSE), ethyl-acetate (ESE), or hexane-soluble (HSE) extracts (10 mg/ml) from raw cladode pulp (raw CP), CP without bacterial inoculum and chemically acidified (CP-CT) and CP fermented with Lactobacillus plantarum 1MR20 (1MR20) and CIL6 (CIL6), Lactobacillus brevis POM4 (POM4), Lactobacillus rossiae 2LC8 (2LC8) and Pediococcus pentosaceus CILSWE5 (CILSWE5). Only the best performing strains and representative species were considered. Data are the means (± SD) of three independent experiments performed in triplicate. Data were subjected to one-way ANOVA; pair-comparison of treatment means was achieved by Tukey’s procedure at P0.05. Bars with different superscript letters differ significantly (P<0.05).