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Lactic Acid Fermentation of Cactus Cladodes (Opuntia ficus-indica L.) Generates Flavonoid Derivatives with Antioxidant and Anti-Inflammatory Properties

Fig 5

Interleukin-8 (IL-8) (A) and tumour necrosis alfa (TNFα) (B) release by Caco-2/TC7 cells. Caco-2/TC7 cells were stimulated for 24 h with interferon-gamma (INF-γ) (2 ng/mL) and subsequently treated with water-soluble (WSE), ethyl-acetate (ESE), or hexane-soluble (HSE) extracts (10 mg/ml) from raw cladode pulp (raw CP), CP without bacterial inoculum and chemically acidified (CP-CT) and CP fermented with Lactobacillus plantarum 1MR20 (1MR20) and CIL6 (CIL6), Lactobacillus brevis POM4 (POM4), Lactobacillus rossiae 2LC8 (2LC8) and Pediococcus pentosaceus CILSWE5 (CILSWE5). Data are the means (± SD) of three independent experiments performed in triplicate. Data were subjected to one-way ANOVA; pair-comparison of treatment means was achieved by Tukey’s procedure at P0.05. Bars with different superscript letters differ significantly (P<0.05).

Fig 5

doi: https://doi.org/10.1371/journal.pone.0152575.g005