Geniposide Protects Primary Cortical Neurons against Oligomeric Aβ1-42-Induced Neurotoxicity through a Mitochondrial Pathway
Fig 7
The effects of geniposide on Aβ1-42-induced release of mitochondrial cytochrome c and caspase-3/9 activity.
Neurons were cultured in the presence of 5 μM oligomeric Aβ1–42 and geniposide (2.5 μM, 5 μM, 10 μM) for 24 h, harvested and the levels of specific proteins were assessed by western blot analysis (A). Levels of cytochrome c in the cytosolic fraction (B) or mitochondrial fractions (C), Geniposide attenuated mitochondrial cytochrome c releasing to cytosolic fraction in neurons induced by oligomeric Aβ1–42 at 5 μM. Caspase-3/9 activity was assessed by microplate fluorometer. The increased activity of caspase-3/9 was attenuated (d, e) by the present of geniposide in a dose-independent manner. NS: non significance. N = 6 per group of cells. Studies were repeated four times and data were expressed as mean ± SEM of percentage of vehicle-treated cells.