Geniposide Protects Primary Cortical Neurons against Oligomeric Aβ1-42-Induced Neurotoxicity through a Mitochondrial Pathway
Fig 6
The effects of geniposide on Aβ1-42-induced mitochondrial abnormalities.
A, Mitochondrial membrane potential. Cells were labeled with JC-1 dye and analyzed by laser-scanning confocal microscope per the manufacturer’s protocol. In the live non-apoptotic cells, the mitochondria appearred following aggregation of JC-1 reagent. In the apoptotic cells, the dye remains in its monomeric form and appears green. Scalebar = 250 μm. B, Quantitative analysis of the Δψm among groups. The ratio of red to green fluorescence in A was used as the indicator for mitochondrial membrane potential. C, The ATP level was determined by fluorescence microplate reader. D, CcO activity was tested by spectrophotometer. NS: non significance. N = 6 per group of cells. Studies were repeated four times and data were expressed as mean ± SEM of percentage of vehicle-treated cells.