Role of the Substrate Specificity-Defining Residues of Human SIRT5 in Modulating the Structural Stability and Inhibitory Features of the Enzyme
Fig 5
Real-time Progress Curve for SIRT5-catalyzed Desuccinylation Reaction.
The time courses of the SIRT5 catalyzed reaction using the fixed concentrations of Ac-Suclys-AMC substrate and NAD+, and varying concentrations of trypsin as the coupling enzyme are shown. The dashed curves represent the time dependent increase in fluorescence at 460 nm (λex = 350 nm) due to release of 7-hydroxyl-4-methylcoumarin (AMC) from the substrate. The solid lines indicate the linear regression analysis of the data after the initial lag phases (the dashed curves).