Role of the Substrate Specificity-Defining Residues of Human SIRT5 in Modulating the Structural Stability and Inhibitory Features of the Enzyme
Fig 4
Thermal Denaturation Profiles of SIRT5 Variants and SIRT1.
The ellipticity of (A) SIRT5 wild type, (B) Y102A, (C) R105I, (D) Y102A/R105I and (E) SIRT1were measured at 208 nm as a function of temperature. The solid smooth lines represent the best fit of the data by the single phase Boltzmann equation (Eq 2) to derive the Tm values. (F) The CD spectra of wild-type and mutant enzymes upon heating at their final melting temperatures followed by cooling at 25°C