Role of the Substrate Specificity-Defining Residues of Human SIRT5 in Modulating the Structural Stability and Inhibitory Features of the Enzyme
Fig 3
Circular Dichroim (CD) Spectra of SIRT5 Variants and SIRT1.
The mean residue ellipticities of 10 μM enzymes as a function of wavelength are shown. The experiments were performed in 5 mM Tris-HCl buffer, pH 7.5 using a quartz cuvette of 1 mm path length.