Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling
Fig 5
Physiologic APN concentrations increased NO production in an AdipoR1 and Cav-1 dependent manner.
NO production in HUVEC medium was determined after incubation with vehicle or APN (5μg/ml) for 15 minutes. Knockdown of AdipoR1 (but not AdipoR2), knockdown of Cav-1, and treatment with L-NAME (100 μmol/L) abolished APN-induced NO production enhancement. n = 6–8 dishes/group. ##, P<0.01 vs respective control. R1, R2 represent AdipoR1 and AdipoR2, respectively.