Ezetimibe Promotes Brush Border Membrane-to-Lumen Cholesterol Efflux in the Small Intestine
Fig 1
Inhibition of NPC1L1 increases trans-intestinal cholesterol efflux (TICE).
A, An example of the TICE assay settings. B, An illustrated protocol for TICE assay. Assay time course is indicated from left to right. Arrow heads show the time points when reagents were given to mice. C, Decay per minute (DPM) counts in 5 μl blood obtained at the indicated time points after 3H-cholesterol intravenous infusion via the jugular vein. The DPM counts for individual mice were normalized against that at 60 min. Each symbol indicates an individual assay result. Infused tracer seemed to be equivalent in the circulation approximately 30 min after the infusion. Open symbols, vehicle controls; closed symbols, mice treated with ezetimibe. D, Ezetimibe treatment increased TICE. Open circles, vehicle; closed circles, 50 μg ezetimibe treated. Plots and error bars show mean and SEM (n = 8), respectively. E, Plots for 3H-DPM counts in sera (left, open circles) and those in total perfusates (right, gray circles). F, Comparison of the area under the concentration-time curve (AUC) of D. Each plot shows an individual assay result. P value was obtained using the Student’s t-test.