A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
Fig 1
Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression.
Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.