Domestication Syndrome Is Investigated by Proteomic Analysis between Cultivated Cassava (Manihot esculenta Crantz) and Its Wild Relatives
Fig 3
Starch staining with KI in storage roots and SEM pictures of starch granules incubated with cell-free supernatants, and paraffin section of transverse and longitude of storage roots of SC205, SC8 and W14.
Paraffin section of transverse and longitude of storage roots of SC205, SC8 and W14, stained with Safranin O/Fast green and viewed under light microscope X20. (a1), (b1) and (c1), transverse sections of cassava genotype W14, SC205 and SC8, respectively; (a2), (b2) and (c2), starch staining with KI of cassava genotype W14, SC205 and SC8, respectively; (a3), (b3) and (c3), SEM of starch granules of cassava W14, SC205 and SC8, respectively. SEM magnification time was 1000; scale bar = 50 μm. (a4)-(c4), transverse sections of storage roots from W14, SC205 and SC8; (a5)-(c5), longitude sections of storage roots from W14, SC205and SC8. Scale bar = 100 μm. It shows more vessel grouping and tyloses with starch granules. SC205 and SC8 showing parenchyma cells with more starch granules, while W14 showing parenchyma cells with a little starch, these cells are bigger. Red arrows indicate xylem vessel, black arrow shows parenchyma cell, and blue arrow presents starch granules.