Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements
Fig 4
Effects of activating additional ATP consumers on glycolytic rate in C2C12 myoblasts.
a: Respiratory (open column sections) and glycolytic (blue column sections) proton production rates after sequential additions as shown of 10 mM glucose, 1 μM rotenone plus 1 μM myxothiazol, 200 μM monensin, 1 μM FCCP, and 1 mM ouabain, calculated using Eq 1. Data are means ± SEM of n = 4 independent biological replicates. Statistical analysis was of glycolytic proton production rates only (blue column sections). w, well; A, B, C, addition ports. b: Lactate accumulation predicted by glycolytic PPR (left) and measured, in the proposed assay for maximum glycolytic capacity. Data are means ± SEM of n = 3 independent biological replicates. n.s.: not significant; **p ≤ 0.01; ***p ≤ 0.005.