Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements
Fig 3
Assay of glycolytic capacity by inhibition of electron transport in C2C12 myoblasts.
Raw traces of (a) oxygen consumption rate (OCR) and (b) extracellular acidification rate (ECAR) after sequential addition using ports A-C of 10 mM glucose, followed by vehicle and then either 2 μg/mL oligomycin (black), or 1 μM rotenone plus 1 μM myxothiazol (red). One representative experiment is shown. c: Respiratory (open column sections) and glycolytic (blue column sections) proton production rates (PPR) of the experiment exemplified in a and b calculated using Eq 1. Data are means ± SEM of n = 4 independent biological replicates. *p ≤ 0.05. Statistical analysis was of glycolytic proton production rates only (blue column sections). w, well; A, B, C, addition ports. These data are replotted after Fig 4, where another addition (in port D) is also shown.