Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements
Fig 2
Increased glycolytic rate following inhibition of the F1FO-ATP synthase in C2C12 myoblasts.
Raw traces of (a) oxygen consumption rate (OCR) and (b) extracellular acidification rate (ECAR) after sequential additions of 10 mM glucose, 2 μg/mL oligomycin, and 100 mM 2-deoxyglucose. One representative experiment is shown. c: Respiratory (open column sections) and glycolytic (blue column sections) proton production rates (PPR) of the experiment exemplified in a and b calculated using Eq 1. Coloured wedges indicate glycolysis under basal conditions (blue) and apparent glycolytic capacity (green), with the difference between these defined as apparent glycolytic reserve. Data are means ± SEM of n = 6 independent biological replicates.