Family of microRNA-146 Regulates RARβ in Papillary Thyroid Carcinoma
Fig 5
Reduction of endogenous RARB mRNA is shown by real-time PCR Taqman assay in the A549 cell line transfected with pcDNA3-miR-146a-5p (A) or pcDNA3-miR-146b-5p (B) or control plasmid. Data are expressed as mean values +/- SEM. Statistical analysis was performed using an unpaired t test (*** p <0.001). Western blotting for RARβ and β-actin (loading control) in the A549 cells transfected with pcDNA3-miR-146a-5p (C), pcDNA3-miR-146b-5p (D) or control plasmid has shown no significant reduction of protein level. Cells were harvested 72 h after transfection; the relative density of bands was quantified by densitometry.