In Vitro Optimization of Enzymes Involved in Precorrin-2 Synthesis Using Response Surface Methodology
Fig 2
UV-visible absorption spectra of products in the tandem enzyme assay.
Precorrin-2 was produced from ALA by the tandem-enzyme reaction system containing purified, recombinant PBGS, PBGD, UROS, SUMT, and SAM (dotted line); precorrin-2 was then converted into sirohydrochlorin by precorrin-2 dehydrogenase in the presence of NAD (solid line).