Combined Treatment of MCF-7 Cells with AICAR and Methotrexate, Arrests Cell Cycle and Reverses Warburg Metabolism through AMP-Activated Protein Kinase (AMPK) and FOXO1
Fig 5
AICAR+MTX treatment blocks the cell cycle.
(A) MCF-7 cells were seeded in 6-well plate at 10 000 cells/well (n = 6/6/6/6). Cells were treated with AICAR and/or MTX for the time indicated. Cells were fixed, permeabilized and charged with propidium iodide. DNA content was analyzed by flow cytometry. The data on day 1 is the average of 3 parallel experiments, while the data for day 6 is the average of 2 parallel experiments. (B) The data on panel A was further analyzed and the ratio between the different stages were calculated and depicted. The data derived from fig 5A. * indicate statistically significant difference between control and treated cells at p<0.05.