Comparative Analysis of Technologies for Quantifying Extracellular Vesicles (EVs) in Clinical Cerebrospinal Fluids (CSF)
Fig 1
(A) Schematic representation of protocol used for the isolation of CSF microvesicles and exosomes. (B) In nanoparticles tracking analysis, light scattered by EVs is captured by digital camera over a series of frames. The rate of the particle movement is then used to calculate particle size using the Stokes—Einstein equation. (C) In tunable resistive pulse sensing, EVs change the electrical resistance as they pass through a pore-based sensor resulting in a resistive pulse signal. Signals obtained from the measurements can then be used to calculate the size, concentration and charge of each particle by correlating the signal back to a set of known standards. (D) In Vesicle flow cytometry, EVs were stained with an optimized concentration of a fluorogenic lipophilic probe, di-8-ANEPPS, and detected on a custom high sensitivity flow cytometer. Vesicle diameter was estimated by comparison to di-8-stained liposomes.