Cecal Ligation and Puncture Results in Long-Term Central Nervous System Myeloid Inflammation
Fig 5
CLP results in patchy perivascular infiltration by monocytes.
Unoperated mice do not demonstrate any CCR2 immunoreactivity in the brain (A). 14 days after sham operation, sham operated mice demonstrate sparse perivascular CCR2 immunoreactivity (B). CLP operated mice, however, demonstrate multiple cortical and subcortical dense patches of CCR2 immunoreactivity (C, white arrows). Highly immunoreactive cells include both elongated perivascular morphologies (E), extravasating cells (F), and punctate perivascular cells (G), all consistent with monocytes. Notable perivascular immunoreactivity is present in isotype stained tissue, likely reflecting cross-reactivity with nonspecific IgG deposition (E, inset).