AAV-Mediated Clarin-1 Expression in the Mouse Retina: Implications for USH3A Gene Therapy
Fig 2
Localization of vector-expressed CLRN1 following intravitreal delivery.
A. Representative image of retinal cross-section showing CLRN1-Venus fusion fluorescence. Strong expression is seen at the OPL, the stratified dendrites within the IPL, and inner retinal neurons. Scale bar: 30 μm. B. Higher magnification view (40X) of CLRN1-Venus expression showing intense fluorescence at the Müller cells and their apical processes at the outer limiting membrane (arrow). C. Low magnification (10X) image showing the localization of CLRN1-Venus expression along the nerve fiber layer and optic nerve extension of ganglion cell axons. D. Localization of vector-expressed CLRN1-HA protein by immunostaining with an anti-HA antibody (40X magnification). Note the expression in numerous Müller cells with fine processes extending between photoreceptors inner segments. An isolated patch of RPE cells with strong labeling of the apical processes is also present (arrow). Abbreviations: ONL, outer nuclear layer; OPL, outer plexiform layer; IPL, inner plexiform layer; NFL, nerve fiber layer.