Serotonin 2B Receptor Antagonism Prevents Heritable Pulmonary Arterial Hypertension
Fig 7
(A) SB204741 inhibits the elevated contractility of mutant microvascular cells in response to TGF-β1. Mutant microvascular smooth muscle cells exhibit a nearly five-fold increase in TGF-β1 induced contractility compared to their WT counterparts after 72 hours of treatment. TGF-β1 induced contractility in mutant microvascular smooth muscle cells is prevented when cells are treated concurrently with SB204741. Mutant cells synthesize (B) and activate (C) higher amounts of TGF-β1 than WT, and neither TGF-β1 synthesis nor activation is changed in mutant cells by SB204741. Values are expressed as mean ± standard error. n = 3–12 per group, *p<0.05 compared to WT, #p<0.05 compared to no treatment. Significance determined by a two-way ANOVA followed by a Holm-Sidak post hoc test.