Mitochondrial Protein PGAM5 Regulates Mitophagic Protection against Cell Necroptosis
Fig 5
PGAM5 fails to stabilize PINK1 in HT-29 cells.
(A) HT-29 cells transduced with shRNA against PGAM5 were treated with TCZ to induce necroptosis. Cell viability was evaluated by the MTT assay. (B) Mitochondria in HeLa cells and HT-29 cells were stained with anti-Tomm20 and mitochondrial morphology was evaluated by confocal. (C) PGAM5 was knocked down by lentiviral shRNA in HeLa, HT-29 and SY5Y cell lines. Then PINK1 mRNA in the control (NS) and Knock-down cells (PGsh) were quantified by RT-PCR. (D) HT-29 and HeLa cells were treated with CCCP for 3 hours and PINK1 was detected by western blot. * indicated full length PINK1 and Δ indicated cleaved PINK1.(E)HT-29 control (NS) and PGAM5 knock-down cells (PGsh) were treated with CCCP for 3 hours followed by western blot for detecting indicated proteins.