Mitochondrial Protein PGAM5 Regulates Mitophagic Protection against Cell Necroptosis
Fig 3
PGAM5 is required for the mitophagy during cell necroptosis.
(A) TEM micrographs of WT and Pgam5 KO MEFs before and after TCZ treatment. Black arrowheads indicate double-membrane structures with encapsulated mitochondria. The black arrow indicates the mitochondria. The white arrows indicate empty vacuoles. (B) Intracellular ROS level was measured by ROS dye CM-H2CFDA for 30 minutes and analyzed by flow cytometry. WT and Pgam5 KO MEFs were treated with TCZ for the indicated time and proteins from cytosolic part (C) or total cell lysates (D) were blotted for the indicated proteins. (E) Confocal photomicrographs of mtKeima-transduced WT and Pgam5 KO MEFs treated with TCZ for 12 hrs after excitation at 450 nm and at 560 nm.