A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice
Fig 5
Morris Water Maze: Retention of Spatial Reference Memory.
A) Compared to control mice (Ctrl), scopolamine-treated mice (SCP) spent less time in the target quadrant during the retention trial on days 14 and 17 and B) in the 40 cm annulus around the platform center on day 14 (n = 10/group). C) The group occupancy plot from the last retention trial (day 17) shows a similar search pattern for both groups. Red and yellow regions represent areas of high occupancy; green and blue represent areas of low occupancy. D) On days 14 and 17 of the retention trials, 129S6/Tg2576 mice spent less time in the target quadrant compared to WT control mice (statistically not significant). E) 129S6/Tg2576 mice spent significantly less time in the 40 cm platform annulus compared to age-matched WT control mice (n = 18-19/group) on days 14 and 17 of the retention trials. F) Occupancy plots obtained from WT and transgenic mice in the last retention trial (day 17) exhibited a similar search pattern for both groups. Mice that showed repeated episodes of extensive floating (> 10 s/trial; >25% of trials) were excluded from analysis for the entire experiment. Data are mean ± SEM; *p < 0.05; **p < 0.01 (Student’s t-test).