Scalable Production of a Multifunctional Protein (TSG-6) That Aggregates with Itself and the CHO Cells That Synthesize It
Fig 5
In vivo half-life of rhTSG-6 proteins in plasma of mice.
(A) rhTSG-6 (50 μg) was injected through the tail vein and the blood was collected at times indicated. After isolation of the plasma, levels of rhTSG-6 proteins were determined by ELISA. Values are mean and SEM from 3 mice. Distribution (t1/2α) and elimination (t1/2β) were calculated by non-linear least squares regression using GraphPad Prism program. Myeloma-derived rhTSG-6 (R&D Systems): t1/2α = 0.15 h, t1/2β = 0.20 h, CHO cell-derived rhTSG-6: t1/2α = 0.08 h, t1/2β = 0.47 h. The results showed a tendency for the CHO cell-derived rhTSG-6 to have a longer half-life. (B) The difference plasma levels at 24 h after infusion of 50 μg rhTSG-6 of the two proteins. Comparison of the two groups was made by Student’s t test with Welch’s correction. Data are presented as mean ± S.E.M. from 6 mice.