G-Protein α-Subunit Gsα Is Required for Craniofacial Morphogenesis
Fig 5
Loss of Gsα in NCCs results in abnormal ossification.
(A-H) Von Kossa and nuclear red staining of heads coronal sections in Wnt1-cre;Gsαf/f mutants and controls from different embryonic stages. The aggregated mesenchymal cell in maxilla are similar between E12.5 Wnt1-cre;Gsαf/f mutant and control (arrows in A and B); however, the ossification region in E14.5 Wnt1-cre;Gsαf/f mutant is larger than that in control (arrows in C and D), and this phenotype are much more severe at later embryonic stages (arrows in E-H). (I-L) Von Kossa staining (I, J) and alcian blue staining (K, L) show the abnormal ossification and malformation of nasal septum cartilage in E17.5 Wnt1-cre;Gsαf/f mutants (arrows in I and K).