Distribution of T Cells in Rainbow Trout (Oncorhynchus mykiss) Skin and Responsiveness to Viral Infection
Fig 5
Quantification of trout CD8+ cells in anterior and posterior skin regions.
Flow cytometry analysis of rainbow trout leukocytes isolated from anterior (A) and posterior (B) sections of the skin were stained with anti-CD8α mAb. For each individual tissue, FSC/SSC profiles are shown (top panels) and gates for lymphoid cells were defined. Bottom panels depict the CD8+ cells among the lymphoid gate (percentages of CD8α+ cells are shown). (C) Mean percentages of CD8α+ cells from three independent experiments are shown (n = 9 fish, mean ± SD). Asterisks indicate mean values in posterior sections significantly lower than values in anterior areas (P < 0.001).