Histological Characterization of the Tumorigenic “Peri-Necrotic Niche” Harboring Quiescent Stem-Like Tumor Cells in Glioblastoma
Fig 5
The localization of SOX2+ HIF-1α+ RNApII-S2P-/low tumor cells is related to their distances from necrotic areas and blood vessels.
a, b: Serial sections of glioblastoma tissue around a large ischemic necrosis. Double immunostaining for HIF-1α/RNApII-S2P (a) and triple immunostaining for SOX2/HIF-1α/RNApII-S2P (b) are shown (SOX2, red; HIF-1α, blue; RNApII-S2P, brown). Arrows in b indicate SOX2+ HIF-1α+ RNApII-S2P-/low tumor cells. NL, large ischemic necrosis; V, blood vessels. Scale bars, 50 μm. c: Relationship between distances from nearest blood vessels and from necrotic areas in SOX2+ HIF-1α+ RNApII-S2P-/low cells. The data from 135 SOX2+ HIF-1α+ RNApII-S2P-/low cells found in 12 cases of glioblastoma are plotted. The line indicates the regression line (y = 0.205x + 17.4, r = 0.34). The gray region represents y ≤ x.