Camphene, a Plant Derived Monoterpene, Exerts Its Hypolipidemic Action by Affecting SREBP-1 and MTP Expression
Fig 11
Effect of camphene on MTP expression.
On day 7, HepG2 cells were incubated with indicated concentrations of camphene for 24 h in DMEM containing 10% (v/v) human LPDS. Panel A. Total cell protein was extracted and 50 μg of protein were separated on a 12% polyacrylamide gel and analyzed by Western immunoblotting using goat anti-MTP as described in Materials and Methods section. Mouse β-actin was used to control for equal loading and normalization. Relative intensity of the bands was quantified by Phosphorimager. Values express the MTP/actin ratio and are calculated by comparison to control samples the value of which is defined as 1. Panel B. A representative Western blot is shown. Panel C. Total RNA was extracted and subjected to Real Time PCR. The results represent the relative MTP mRNA normalized to endogenous YWHAZ mRNA. The same results were observed after normalization to the housekeeping gene actin (data not shown). The abundance of mRNA in the control-untreated cells was set at 1. Data represent means ± SD of three independent experiments. p<0.001 (***) and p<0.01 (**) vs control cells by the Student-Newmann-Keuls test.