Camphene, a Plant Derived Monoterpene, Exerts Its Hypolipidemic Action by Affecting SREBP-1 and MTP Expression
Fig 3
Effect of camphene, mevinolin and atorvastatin on the incorporation of [14C]-acetate into de novo synthesized triglycerides.
On day 7, HepG2 cells were incubated for 24 h with each compound in medium containing 10% (v/v) human LPDS. The cells received camphene, mevinolin and atorvastatin at the concentrations indicated. Four hours prior to the end of the incubation period, the cells were pulse-labeled with [14C]-acetic acid, sodium salt. The incorporation of acetate into triglycerides was determined as described in Materials and Methods section. Results were normalized to the amount of cellular protein and expressed in percent of the respective control incubations. Results are from three independent experiments in triplicates and expressed as the mean ± SD. Significantly different compared to control: p<0.05 (*); p<0.01 (**); p<0.001 (***); p>0.05 and ns (non significant) vs control by Student-Newmann-Keuls test.