Camphene, a Plant Derived Monoterpene, Exerts Its Hypolipidemic Action by Affecting SREBP-1 and MTP Expression
Fig 2
Effect of camphene, mevinolin and atorvastatin on the incorporation of acetate into de novo synthesized cholesterol.
On day 7, the cells were incubated with the compounds for 24 h in DMEM containing 10% (v/v) human LPDS. Camphene, mevinolin and atorvastatin were received at the concentrations indicated. Four hours prior to the end of the incubation period, the cells were pulse-labeled with [14C]-acetic acid, sodium salt. The incorporation of acetate into cholesterol was determined as described. Results were normalized to the amount of cellular protein and expressed in percent of the respective control incubations. Results are from three independent experiments in triplicates and expressed as the mean ± SD. Significantly different compared to control: p<0.05 (*); p<0.01(**); p<0.001 (***) vs control by Student-Newmann-Keuls test. 25 μM vs 50 μM p<0.05; 25 μM vs 100 μM p<0.01; 50 μM vs 100 μM p>0.05.