Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased β1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding
Fig 5
Upregulation of Mgat5 and β1,6GlcNAc-branched N-glycosylation in myofibroblastic RPE.
(A) Total cellular RNA from native (N) and cultured, myofibroblastic human RPE cells (M) was analysed for β1,6-N-acetylglucosaminyltransferase (Mgat5) expression levels by quantitative real-time RT-PCR as described in the Materials and Methods Section. Data are presented as relative Mgat5 mRNA expression levels as compared with native RPE cells and represent the mean ± SD of at least 3 experiments. (B) Equal amounts of whole cellular protein lysates (50 μg) derived from native (N) and cultured, myofibroblastic (M) human RPE cells of passage 3 were separated by 10% SDS PAGE and Mgat5 levels detected by Western blot. Equal loading was confirmed by GAPDH. Experiments were repeated at least three times