Long Lasting Microvascular Tone Alteration in Rat Offspring Exposed In Utero to Maternal Hyperglycaemia
Fig 3
Western blot analysis of contractile proteins.
(A) Activity of MLC reflected by the pMLC/MLC ratio; (B) activity of PKA reflected by the pPKA/PKA ratio; (C) activity of RhoA reflected by the RhoA-GTP/RhoA ratio; (D) activity of MAP kinase P38 reflected by the ratio of pP38/P38 and protein expression levels of (E) filamin-1 and (F) profilin-1, normalised to β-actin were measured in mesenteric arteries from 3- and 18-month old CMO and DMO. Values are mean ± SEM (n = 5 minimum per group). * p<0.05 and ** p<0.01 DMO vs. CMO at the same age, ## p<0.05 18 vs. 3 months of age.