Aberrant Wound Healing in an Epidermal Interleukin-4 Transgenic Mouse Model of Atopic Dermatitis
Fig 5
Collagen synthesis and deposition.
a) Representative microscopic images of Masson’s Trichrome staining of collagen at days 10, 14 and 21 post-wounding as well as unwounded skin. Blue: stained collagen. b) Corresponding enlarged inserts of a). c) Quantification of blue stained collagen by ImageJ. # p<0.01 and ** p<0.001 compared to IL-4 Tg mice. d) mRNA expression of collagen I and III. * p<0.05 compared to unwounded skin, # p<0.01 compared to IL-4 Tg mice. e) Microscopic images of picrosirious red staining of mature (collagen I, red/orange) and immature collagen (collagen III, green). f) Percent of mature and immature collagen based on picrosirious red staining. # p<0.01 compared to the other group. The number of mice used at each time point was 5.