Mutation of the CH1 Domain in the Histone Acetyltransferase CREBBP Results in Autism-Relevant Behaviors in Mice
Fig 2
CBPΔCH1/ΔCH1 mice show repetitive behaviors, hyperactivity, and less anxiety.
(A,B) CBPΔCH1/ΔCH1 mice display repetitive forelimb movements. White dots (A) indicate position of paws. Scores assigned in (B) represent the frequency of the repetitive movements. 0 = no forelimb repetitive movements (FRM); 1 = occasional FRM; 2 = continuous FRM. Mean ± SEM. N = 8 wild type (WT), 9 CBP+/ΔCH1, 8 CBPΔCH1/ΔCH1. (C) CBPΔCH1/ΔCH1 mice show significantly increased self-grooming time. N = 14 WT, 21 CBP+/ΔCH1, 13 CBPΔCH1/ΔCH1. (D-F) CBPΔCH1/ΔCH1 mice show increased travel distance (D), speed (E), and rearing (F) in a 30-min open field test. (G) CBPΔCH1/ΔCH1 mice stay longer in the center of the open field arena. (H-I) CBPΔCH1/ΔCH1 mice stay shorter in the closed arm, and enter less frequently the closed arm of an elevated plus maze. For (D-I), N = 19 WT, 24 CBP+/ΔCH1, 16 CBPΔCH1/ΔCH1. For (B-I) Asterisks indicate the p value for either Dunnett’s (in the repetitive movement assay) or Tukey (in the other tests) post hoc analysis after one-way ANOVA (*: p<0.05; **: p<0.01; ***: p<0.001; ****: p< 0.0001). All the other pairings are not statistically different.